Journal: Acta Pharmacologica Sinica

Article Title: Co-delivery of docetaxel and silibinin using pH-sensitive micelles improves therapy of metastatic breast cancer

doi: 10.1038/aps.2017.74

Figure Lengend Snippet: Characterization of DSM. (A) The particle size distribution of DSM. (B) TEM image of DSM. Scale bar=200 nm. (C) Fluorescence profiles of Nile red-loading BDP micelles at different pH. (D) In vitro release profiles of DTX and SIL from DSM in pH 7.4 and pH 5.5 media with 1% Tween-80. Data are presented as the mean±SD (n=3).

Article Snippet: Materials DTX was obtained from Knowshine (Shanghai) Pharmachemicals, Inc. SIL was purchased from the Aladdin Reagent Co, Ltd (purity ≥98%, Shanghai, China).

Techniques: Fluorescence, In Vitro

Journal: Acta Pharmacologica Sinica

Article Title: Co-delivery of docetaxel and silibinin using pH-sensitive micelles improves therapy of metastatic breast cancer

doi: 10.1038/aps.2017.74

Figure Lengend Snippet: Cellular internalization of free drug and the BDP micelle in 4T1 cells after 2 h incubation. Nuclei were stained with Hoechst 33342 (blue). DTX and SIL were replaced with Coumarin-6 (green).

Article Snippet: Materials DTX was obtained from Knowshine (Shanghai) Pharmachemicals, Inc. SIL was purchased from the Aladdin Reagent Co, Ltd (purity ≥98%, Shanghai, China).

Techniques: Incubation, Staining

Journal: Acta Pharmacologica Sinica

Article Title: Co-delivery of docetaxel and silibinin using pH-sensitive micelles improves therapy of metastatic breast cancer

doi: 10.1038/aps.2017.74

Figure Lengend Snippet: In vitro cytotoxicity effects of free SIL, free DTX, DM, DSM and BM at different concentrations on 4T1 cells after a 24 h incubation. Data are presented as the mean±SD (n=3). *P<0.05, ***P<0.001.

Article Snippet: Materials DTX was obtained from Knowshine (Shanghai) Pharmachemicals, Inc. SIL was purchased from the Aladdin Reagent Co, Ltd (purity ≥98%, Shanghai, China).

Techniques: In Vitro, Incubation

Journal: Acta Pharmacologica Sinica

Article Title: Co-delivery of docetaxel and silibinin using pH-sensitive micelles improves therapy of metastatic breast cancer

doi: 10.1038/aps.2017.74

Figure Lengend Snippet: In vivo anti-metastasis effect in 4T1 tumor-bearing mice. (A, B) Images of the lungs (A) and H&E staining of the lung sections (B) at the end of the experiment (1: saline; 2: BM; 3: SIL+DTX; 4: DSM). (C) Quantitative analysis of the pulmonary metastatic nodules. Data are presented as the mean±SD (n=5). **P<0.01.

Article Snippet: Materials DTX was obtained from Knowshine (Shanghai) Pharmachemicals, Inc. SIL was purchased from the Aladdin Reagent Co, Ltd (purity ≥98%, Shanghai, China).

Techniques: In Vivo, Staining, Saline